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KMID : 0644320010070010061
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Journal of Korean Oriental Oncology
2001 Volume.7 No. 1 p.61 ~ p.75
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Experimental Study of WhaYoungJiTongTang(Heyingjitongtang)on the anti-angionesis
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Kim In-Seok
Park Jun-Hyuk
Kang Hee
Kim Sung-Hoon
Yu Young-Beob
Shim Bum-Sang
Choi Seung-Hun
Ahn Kyoo-Suk
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Abstract
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The purpose of this study is to prove the angiogenesis effects of WhaYoungJiTongTang (hereinafter referred to as the ’WYJTT’). For the study, by utilizing liver cancer cell line; SK-HEP-1, lung cancer cell line: A549, stomach cancer cell line: AGS and bovine capillary endothelial cell: BCE, the effects of the WYJTT on toxicity and proliferation ability of cells and the effects on anti-angiogenesis of bovine capillary endothelial cell and of mice’s aorta were studied. 1. Cell viability assay In comparison with the control group, when 100ug/ml of WYJTT was injected, the viability was reduced in SK-Hep-1, 400ug/ml in A549 and 200ug/ml in AGS. 2. Cell proliferation assay In comparison with the control group, when 600ug/ml of WYITT was injected, DNA synthesis was reduced to 35.1% in the SK-Hep-1, 56.0% in A549, and 25.8% in BCE (bovine capillary endothelial cell); and when 400ug/ml was injected, DNA synthesis was reduced to 12.1 in AGS. 3. Tube formation assay In the event that BCE is injected with WYJTT in each of its content gradient, the anti-angiogenesis was effective in amounts of 400ug/ml with 6 hours of the treatment. 4. Aortic ring assay In comparison with the control group, the angiogenesis was restricted to the remarkable degree in amount of 200ug/ml: 10% in 400ug/ml; and fully inhibited in each of 800ug/ml and 1600ug/ml. As a result of the experiments mentioned above, WYJTT showed its anti-angiogenesis effects against cancer cell line.
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KEYWORD
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